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AsurDx™ Fast qPCR UNG Master Mix

The AsurDxTM Fast qPCR UNG Master Mix is a ready-to-use reagent for rapid, probe-based real-time PCR. It features a fast-acting hot-start DNA polymerase, UNG enzyme, and inhibitor-resistant buffer with dNTPs (including UTP) – supporting accurate results in as little as 30 minutes. Designed to reduce false positives and perform reliably across complex samples, it’s compatible with major qPCR platforms including ABI, Bio-Rad, Roche, and Eppendorf.

Features of Fast qPCR UNG Master Mix

  • Supports both fast and standard PCR cycling, with results in as little as 30 minutes
  • UNG system with dUTP minimizes carryover contamination
  • Tolerates PCR inhibitors commonly found in animal samples
  • Delivers high specificity and sensitivity for low-copy target detection
  • Compatible with major qPCR platforms including ABI, Bio-Rad, Roche, and Eppendorf

*Box images are representative, size and labels may vary.

*Warning: Read the Safety Data Sheets (SDSs) and follow the handling instructions. Wear appropriate protective eyewear, clothing, and gloves.  For Research Use Only. Not for use in diagnostic procedures.

Reagent Composition

Supplied as a 2X master mix (probe-based):

  • Hot-Start DNA Polymerase
  • Uracil-N-Glycosylase (UNG)
  • PCR Buffer
  • MgCl2
  • dNTPs (including dUTP)
  • Stabilizer

Storage

The kit is transported on wet ice. All components should be kept at -20℃ for long-term storage and 4℃ for up to 3 months.  Please mix thoroughly after thawing and centrifuge before using. Avoid frequent freeze-thaw.

Precautions

  • For amplification of longer fragments (>200 bp) or primers with low annealing temperatures, use a three-step method
  • Optimize probe/primer concentrations as needed
  • Avoid opening PCR tubes post-run to reduce contamination risk
  • Use separate pipettes and workspace for pre- and post-PCR steps
Product NameReactionCatalog NumberDescription
AsurDxTM Fast qPCR UNG Master Mix50MM-2502P-UNG2x probe-based master mix with hot-start DNA Polymerase, UNG, dNTPs, PCR Buffer, MgCl2, stabilizer.
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