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AsurDx™ Fast qRT-PCR UNG Master Mix

The AsurDxTM Fast qRT-PCR UNG Master Mix is a one-step, probe-based reagent for reverse transcription and real-time PCR. Designed for direct amplification from crude samples without RNA extraction, it contains a blend of RT and DNA polymerases, UNG, and an optimized buffer system with dNTPs (including dUTP) to streamline high-throughput testing. Compatible with major qPCR platforms including ABI, Bio-Rad, Eppendorf, and Roche.

Features of Fast qRT-PCR UNG Master Mix

  • Without the need of RNA extraction, this kit enables one-step qRT-PCR
  • Includes UNG + dUTP system to minimize carryover contamination
  • Optimized buffer enhances tolerance to PCR inhibitors
  • Delivers robust results from a variety of samples, including anticoagulated whole blood, plasma, serum, swabs, and stools
  • Fast cycling capability; complete reactions in as little as 30 minutes

*Box images are representative, size and labels may vary.

*Warning: Read the Safety Data Sheets (SDSs) and follow the handling instructions. Wear appropriate protective eyewear, clothing, and gloves.  For Research Use Only. Not for use in diagnostic procedures.

Reagent Composition

Supplied as 2-part system:

  • 10x Fast RT-PCR UNG Mix
  • 2x Fast RT-PCR UNG Buffer

Storage

The kit is transported on wet ice. All components should be kept at -20℃ for long-term storage and 4℃ for up to 3 months.  Please mix thoroughly after thawing and centrifuge before using. Avoid frequent freeze-thaw.

Precautions

  • For amplification of longer fragments (>200 bp) or primers with low annealing temperatures, use a three-step method
  • Optimize probe/primer concentrations as needed
  • Avoid opening PCR tubes post-run to reduce contamination risk
  • Use separate pipettes and workspace for pre- and post-PCR steps
Product NameReactionCatalog NumberDescription
AsurDxTM Fast qRT-PCR UNG Master Mix50MM-2501P-RTOne-step master mix for direct qRT-PCR from crude samples. Includes rapid DNA polymerase, RTase, TS-UNG, dNTPs (with dUTP), and inhibitor-resistant components.
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