AsurDx™ Actinobacillus pleuropneumoniae (APP) Antibody Test
The AsurDxTM Actinobacillus pleuropneumoniae (APP) Antibody Test Kit is designed for the detection of pig antibodies specific to Actinobacillus pleuropneumoniae (APP).
- Detects the antibodies against all APP serotypes in pig serum/plasma;
- Possible to differentiate infected animals (positive test result) from vaccinated animals (negative test result) (DIVA);
- Procedures last less than 75 minutes;
- Provides a simple, rapid, sensitive and cost-effective enzyme-based immunoassay (ELISA) screening method
*Availability/Distribution: Product is designed and developed by BioStone US Texas headquarter and manufactured/assembled by BioStone oversea subsidiaries or partners. Currently, the product is only available outside of the USA. Regulatory requirements vary by oversea countries; the product may not be available in your geographic area.
|Coated Antigen||APP antigen-coated Plate|
|Incubation Time||75 minutes|
|Storage||At least 12 months|
|Specificity||APP all serogroup-specific pig antibodies|
|Plates||2 plates||5 plates|
|Plate Format||12 X 8-well strips||12 X 8-well strips|
Porcine pleuropneumonia is a severe and contagious respiratory disease, primarily of young pigs (≤6 momths old). The causal organism is Actinobacillus pleuropneumoniae (APP). APP is a hemolytic, Gram-negative, capsulated, coccobacillary rod, highly host-specific for swine. Fifteen serotypes have been identified; they vary widely in virulence and significance across countries. Historically, serotypes 1, 5, and 7 have been prevalent in the USA.
Clinical Signs vary considerably between acute and chronic forms of APP. In acute outbreaks, sudden deaths are common. Clinical signs of the acute disease are dyspnea, coughing, anorexia, depression, fever and sometimes vomiting. Peak mortality usually occurs in pigs 10-16 weeks old. Mortality can reach 20-80% in fattening pigs. Abortions may occur in acutely infected pregnant females. Chronic cases may become apparent following an acute outbreak. Chronic cough and slow growth then are the usual signs due to the presence of pleural adhesions and abscesses that form in recovered lungs.
The APP organism secretes 4 exotoxins, ApxI, ApxII, ApxIII and ApxIV, together called RTX toxins, and produces abundant endotoxin. There are many lines of evidence that suggest these toxins play a predominant role in the pathogenicity of APP with different activities. Because of their strong antigenic properties, they have been used in the serodiagnosis of APP infections. However, other bacteria express toxins that serologically cross-react with ApxI, ApxII or ApxIII. In contrast, ApxIV, a fourth RTX determinant, has been found to be common and specific to all APP serotypes.