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AsurDx™ ELISA Blocking Buffers
Biostone Animal Health AsurDxTM blocking buffers can block excess binding sites in ELISA. These blocking buffers reduce or eliminate many of the problems encountered with traditional blocking reagents, such as cross-reactivity and interference from glycosylation. Additionally, AsurDxTM blocking buffers are compatible with antibodies and avidin/biotin systems. Some of the blocking buffers contain the detergent Tween 20, which improves blocking performance in many detection systems.
*Warning: Read the Safety Data Sheets (SDSs) and follow the handling instructions. Wear appropriate protective eyewear, clothing, and gloves. For Research Use Only. Not for use in diagnostic procedures.
Product List
| Product Name | Volume | Catalog Number | Description | Storage |
|---|---|---|---|---|
| AsurDxTM ELISA Blocking Buffer I | 60mL | AD3010C-60 | 2% casein solution: Dissolve casein in deionized water to make a 2% casein solution, add 0.2% Kathon, pH 7.4. | 4°C |
| AsurDxTM ELISA Blocking Buffer I | 30mL | AD3010C-30 | 2% casein solution: Dissolve casein in deionized water to make a 2% casein solution, add 0.2% Kathon, pH 7.4. | 4°C |
| AsurDxTM ELISA Blocking Buffer I | 15mL | AD3010C-15 | 2% casein solution: Dissolve casein in deionized water to make a 2% casein solution, add 0.2% Kathon, pH 7.4. | 4°C |
| AsurDxTM ELISA Blocking Buffer I | 10mL | AD3010C-10 | 2% casein solution: Dissolve casein in deionized water to make a 2% casein solution, add 0.2% Kathon, pH 7.4. | 4°C |
| AsurDxTM ELISA Blocking Buffer II | 60mL | AD3020R | Tris buffer containing 2% casein (red): Dissolve casein in Tris buffer to make a solution with 2% casein, add 0.2% Kathon, pH 7.4, red food dye. | 4°C |
| AsurDxTM ELISA Blocking Buffer III | 60mL | AD3030G | Tris buffer containing 2% casein (green): Dissolve casein in Tris buffer with 2% casein, add 0.05% Tween-20, 0.2% Kathon, pH 7.4, green food dye. | 4°C |
| AsurDxTM ELISA Blocking Buffer IV | 60mL | AD3040B-60 | 2% casein solution: Dissolve casein in deionized water with 1% Vitamin C, 0.05% Tween-20, 0.2% Kathon, pH 7.4. Packed in brown bottle. | 4°C |
| AsurDxTM ELISA Blocking Buffer IV | 30mL | AD3040B-30 | 2% casein solution: Dissolve casein in deionized water with 1% Vitamin C, 0.05% Tween-20, 0.2% Kathon, pH 7.4. Packed in brown bottle. | 4°C |
| AsurDxTM ELISA Blocking Buffer IV | 15mL | AD3040B-15 | 2% casein solution: Dissolve casein in deionized water with 1% Vitamin C, 0.05% Tween-20, 0.2% Kathon, pH 7.4. Packed in brown bottle. | 4°C |
| AsurDxTM ELISA Blocking Buffer IV | 10mL | AD3040B-10 | 2% casein solution: Dissolve casein in deionized water with 1% Vitamin C, 0.05% Tween-20, 0.2% Kathon, pH 7.4. Packed in brown bottle. | 4°C |
| AsurDxTM ELISA Blocking Buffer V | 260mL | AD3260C | Phosphate buffer containing 2% casein: Dissolve casein in phosphate buffer with 2% casein, add 0.2% Kathon, pH 7.4. | 4°C |
| AsurDxTM ELISA Blocking Buffer VI | 600mL | AD3600C | Phosphate buffer containing 2% casein (with Tween): Dissolve casein in phosphate buffer with 2% casein, add 0.05% Tween-20, 0.2% Kathon, pH 7.4. | 4°C |
| AsurDxTM ELISA Blocking Buffer H | 60 mL | AD3010H-60 | 1% casein solution: Dissolve casein in deionized water with 1% Vitamin C, 0.05% Tween-20, 0.2% Kathon, pH 7.4. Packed in brown bottle. | 4°C |
| AsurDxTM ELISA Blocking Buffer H | 30 mL | AD3010H-30 | 1% casein solution: Dissolve casein in deionized water with 1% Vitamin C, 0.05% Tween-20, 0.2% Kathon, pH 7.4. Packed in brown bottle. | 4°C |
| AsurDxTM ELISA Blocking Buffer H | 15 mL | AD3010H-15 | 1% casein solution: Dissolve casein in deionized water with 1% Vitamin C, 0.05% Tween-20, 0.2% Kathon, pH 7.4. Packed in brown bottle. | 4°C |
| AsurDxTM ELISA Blocking Buffer H | 10 mL | AD3010H-10 | 1% casein solution: Dissolve casein in deionized water with 1% Vitamin C, 0.05% Tween-20, 0.2% Kathon, pH 7.4. Packed in brown bottle. | 4°C |
| AsurDxTM ELISA Blocking Buffer M | 120 mL | AD3120C-120 | Phosphate buffer containing 1% casein: Dissolve casein in phosphate buffer with 1% casein, add 1% Vitamin C, 0.05% Tween-20, 0.2% Kathon, pH 7.4. Packed in brown bottle. | 4°C |
| AsurDxTM ELISA Blocking Buffer M | 30 mL | AD3120C-30 | Phosphate buffer containing 1% casein: Dissolve casein in phosphate buffer with 1% casein, add 1% Vitamin C, 0.05% Tween-20, 0.2% Kathon, pH 7.4. Packed in brown bottle. | 4°C |
| AsurDxTM ELISA Blocking Buffer M | 15 mL | AD3120C-15 | Phosphate buffer containing 1% casein: Dissolve casein in phosphate buffer with 1% casein, add 1% Vitamin C, 0.05% Tween-20, 0.2% Kathon, pH 7.4. Packed in brown bottle. | 4°C |
| AsurDxTM ELISA Blocking Buffer M | 10 mL | AD3120C-10 | Phosphate buffer containing 1% casein: Dissolve casein in phosphate buffer with 1% casein, add 1% Vitamin C, 0.05% Tween-20, 0.2% Kathon, pH 7.4. Packed in brown bottle. | 4°C |
| AsurDxTM ELISA Blocking Buffer N | 30 mL | AD3220N-30 | Phosphate buffer containing 3% casein: Dissolve casein in phosphate buffer with 3% casein, add 1% Vitamin C, 0.05% Tween-20, 0.2% Kathon, pH 7.4. Mix well, filter through 0.2 μm membrane, packed in brown bottle. | 4°C |
| AsurDxTM ELISA Blocking Buffer N | 15 mL | AD3220N-15 | Phosphate buffer containing 3% casein: Dissolve casein in phosphate buffer with 3% casein, add 1% Vitamin C, 0.05% Tween-20, 0.2% Kathon, pH 7.4. Mix well, filter through 0.2 μm membrane, packed in brown bottle. | 4°C |
| AsurDxTM ELISA Blocking Buffer N | 10 mL | AD3220N-10 | Phosphate buffer containing 3% casein: Dissolve casein in phosphate buffer with 3% casein, add 1% Vitamin C, 0.05% Tween-20, 0.2% Kathon, pH 7.4. Mix well, filter through 0.2 μm membrane, packed in brown bottle. | 4°C |
Block ELISA Plates
1.Coat the ELISA plate with antigen or antibody.
2.Add 300 µL of AsurDxTM blocking buffers to each well, then incubate the plate for 1 hour at room temperature or 37°C. Alternatively, add 300 μL of blocking buffer to each well, then immediately invert the plate to empty contents. Repeat this process two more times.
3.Proceed with the ELISA protocol that is appropriate for your downstream detection. For storage, invert plate for approximately 2 hours to dry. Transfer plate to a plastic bag or other container containing a desiccant, such as silica gel. Store the plate at 4°C.