AsurDx™ ELISA Blocking Buffers
Biostone Animal Health AsurDxTM blocking buffers can block excess binding sites in ELISA. These blocking buffers reduce or eliminate many of the problems encountered with traditional blocking reagents, such as cross-reactivity and interference from glycosylation. Additionally, AsurDxTM blocking buffers are compatible with antibodies and avidin/biotin systems. Some of the blocking buffers contain the detergent Tween 20, which improves blocking performance in many detection systems.
*Warning: Read the Safety Data Sheets (SDSs) and follow the handling instructions. Wear appropriate protective eyewear, clothing, and gloves. For Research Use Only. Not for use in diagnostic procedures.
|Product Name||Volume||Catalog Number||Description||Storage|
|AsurDxTM ELISA Blocking Buffer I||60mL||AD3010C||Single purified protein in Tris-buffered saline, pH 7.4, 0.2% Kathon.||4°C|
|AsurDxTM ELISA Blocking Buffer II||60mL||AD3020R||Protein-Free compound in Tris-buffered saline, 0.2% Kathon, pH 7.4 with red food color.||4°C|
|AsurDxTM ELISA Blocking Buffer III||60mL||AD3030G||Protein-Free T20 (TBS) Blocking Buffer, protein-free compound in Tris-buffered saline, pH 7.4 with 0.05% Tween 20 Detergen,0.2% Kathon and green food color||4°C|
|AsurDxTM ELISA Blocking Buffer IV||60mL||AD3040B||Protein-Free (PBS) Blocking Buffer, protein-free compound in phosphate-buffered saline, pH 7.4, 0.2% Kathon in brown color bottle.||4°C|
|AsurDxTM ELISA Blocking Buffer V||260mL||AD3260C||Single purified protein in phosphate-buffered saline, pH 7.4,0.2% Kathon.||4°C|
|AsurDxTM ELISA Blocking Buffer VI||600mL||AD3600C||Single purified protein in phosphate-buffered saline,pH 7.4 with 0.05% Tween 20 Detergent,0.2% Kathon.||4°C|
|AsurDxTM ELISA Blocking Buffer H||60 mL||AD3010H||3% purified casein protein in phosphate-buffered saline, pH 7.4 with 0.05% Tween 20 Detergent,0.2% Kathon, brown food color.||4°C|
|AsurDxTM ELISA Blocking Buffer M||120 mL||AD3120C||2% purified casein protein in phosphate-buffered saline, pH 7.4 with 0.05% Tween 20 Detergent,0.2% Kathon.||4°C|
Block ELISA Plates
1.Coat the ELISA plate with antigen or antibody.
2.Add 300 µL of AsurDxTM blocking buffers to each well, then incubate the plate for 1 hour at room temperature or 37°C. Alternatively, add 300 μL of blocking buffer to each well, then immediately invert the plate to empty contents. Repeat this process two more times.
3.Proceed with the ELISA protocol that is appropriate for your downstream detection. For storage, invert plate for approximately 2 hours to dry. Transfer plate to a plastic bag or other container containing a desiccant, such as silica gel. Store the plate at 4°C.