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AsurDx™ ELISA Blocking Buffers

Biostone Animal Health AsurDxTM blocking buffers can block excess binding sites in ELISA. These blocking buffers reduce or eliminate many of the problems encountered with traditional blocking reagents, such as cross-reactivity and interference from glycosylation. Additionally, AsurDxTM blocking buffers are compatible with antibodies and avidin/biotin systems. Some of the blocking buffers contain the detergent Tween 20, which improves blocking performance in many detection systems.

*Warning: Read the Safety Data Sheets (SDSs) and follow the handling instructions. Wear appropriate protective eyewear, clothing, and gloves.  For Research Use Only. Not for use in diagnostic procedures.


Product List

Product NameVolumeCatalog NumberDescriptionStorage
AsurDxTM ELISA Blocking Buffer I60mLAD3010C-602% casein solution: Dissolve casein in deionized water to make a 2% casein solution, add 0.2% Kathon, pH 7.4.4°C
AsurDxTM ELISA Blocking Buffer I30mLAD3010C-302% casein solution: Dissolve casein in deionized water to make a 2% casein solution, add 0.2% Kathon, pH 7.4.4°C
AsurDxTM ELISA Blocking Buffer I15mLAD3010C-152% casein solution: Dissolve casein in deionized water to make a 2% casein solution, add 0.2% Kathon, pH 7.4.4°C
AsurDxTM ELISA Blocking Buffer I10mLAD3010C-102% casein solution: Dissolve casein in deionized water to make a 2% casein solution, add 0.2% Kathon, pH 7.4.4°C
AsurDxTM ELISA Blocking Buffer II60mLAD3020RTris buffer containing 2% casein (red): Dissolve casein in Tris buffer to make a solution with 2% casein, add 0.2% Kathon, pH 7.4, red food dye.4°C
AsurDxTM ELISA Blocking Buffer III60mLAD3030GTris buffer containing 2% casein (green): Dissolve casein in Tris buffer with 2% casein, add 0.05% Tween-20, 0.2% Kathon, pH 7.4, green food dye.4°C
AsurDxTM ELISA Blocking Buffer IV60mLAD3040B-602% casein solution: Dissolve casein in deionized water with 1% Vitamin C, 0.05% Tween-20, 0.2% Kathon, pH 7.4. Packed in brown bottle.4°C
AsurDxTM ELISA Blocking Buffer IV30mLAD3040B-302% casein solution: Dissolve casein in deionized water with 1% Vitamin C, 0.05% Tween-20, 0.2% Kathon, pH 7.4. Packed in brown bottle.4°C
AsurDxTM ELISA Blocking Buffer IV15mLAD3040B-152% casein solution: Dissolve casein in deionized water with 1% Vitamin C, 0.05% Tween-20, 0.2% Kathon, pH 7.4. Packed in brown bottle.4°C
AsurDxTM ELISA Blocking Buffer IV10mLAD3040B-102% casein solution: Dissolve casein in deionized water with 1% Vitamin C, 0.05% Tween-20, 0.2% Kathon, pH 7.4. Packed in brown bottle.4°C
AsurDxTM ELISA Blocking Buffer V260mLAD3260CPhosphate buffer containing 2% casein: Dissolve casein in phosphate buffer with 2% casein, add 0.2% Kathon, pH 7.4.4°C
AsurDxTM ELISA Blocking Buffer VI600mLAD3600CPhosphate buffer containing 2% casein (with Tween): Dissolve casein in phosphate buffer with 2% casein, add 0.05% Tween-20, 0.2% Kathon, pH 7.4. 4°C
AsurDxTM ELISA Blocking Buffer H60 mLAD3010H-601% casein solution: Dissolve casein in deionized water with 1% Vitamin C, 0.05% Tween-20, 0.2% Kathon, pH 7.4. Packed in brown bottle.4°C
AsurDxTM ELISA Blocking Buffer H30 mLAD3010H-301% casein solution: Dissolve casein in deionized water with 1% Vitamin C, 0.05% Tween-20, 0.2% Kathon, pH 7.4. Packed in brown bottle.4°C
AsurDxTM ELISA Blocking Buffer H15 mLAD3010H-151% casein solution: Dissolve casein in deionized water with 1% Vitamin C, 0.05% Tween-20, 0.2% Kathon, pH 7.4. Packed in brown bottle.4°C
AsurDxTM ELISA Blocking Buffer H10 mLAD3010H-101% casein solution: Dissolve casein in deionized water with 1% Vitamin C, 0.05% Tween-20, 0.2% Kathon, pH 7.4. Packed in brown bottle.4°C
AsurDxTM ELISA Blocking Buffer M120 mLAD3120C-120Phosphate buffer containing 1% casein: Dissolve casein in phosphate buffer with 1% casein, add 1% Vitamin C, 0.05% Tween-20, 0.2% Kathon, pH 7.4. Packed in brown bottle.4°C
AsurDxTM ELISA Blocking Buffer M30 mLAD3120C-30
Phosphate buffer containing 1% casein: Dissolve casein in phosphate buffer with 1% casein, add 1% Vitamin C, 0.05% Tween-20, 0.2% Kathon, pH 7.4. Packed in brown bottle.4°C
AsurDxTM ELISA Blocking Buffer M15 mLAD3120C-15Phosphate buffer containing 1% casein: Dissolve casein in phosphate buffer with 1% casein, add 1% Vitamin C, 0.05% Tween-20, 0.2% Kathon, pH 7.4. Packed in brown bottle.4°C
AsurDxTM ELISA Blocking Buffer M10 mLAD3120C-10Phosphate buffer containing 1% casein: Dissolve casein in phosphate buffer with 1% casein, add 1% Vitamin C, 0.05% Tween-20, 0.2% Kathon, pH 7.4. Packed in brown bottle.4°C
AsurDxTM ELISA Blocking Buffer N30 mLAD3220N-30Phosphate buffer containing 3% casein: Dissolve casein in phosphate buffer with 3% casein, add 1% Vitamin C, 0.05% Tween-20, 0.2% Kathon, pH 7.4. Mix well, filter through 0.2 μm membrane, packed in brown bottle.4°C
AsurDxTM ELISA Blocking Buffer N15 mLAD3220N-15Phosphate buffer containing 3% casein: Dissolve casein in phosphate buffer with 3% casein, add 1% Vitamin C, 0.05% Tween-20, 0.2% Kathon, pH 7.4. Mix well, filter through 0.2 μm membrane, packed in brown bottle.4°C
AsurDxTM ELISA Blocking Buffer N10 mLAD3220N-10Phosphate buffer containing 3% casein: Dissolve casein in phosphate buffer with 3% casein, add 1% Vitamin C, 0.05% Tween-20, 0.2% Kathon, pH 7.4. Mix well, filter through 0.2 μm membrane, packed in brown bottle.4°C

Block ELISA Plates

1.Coat the ELISA plate with antigen or antibody.

2.Add 300 µL of AsurDxTM blocking buffers to each well, then incubate the plate for 1 hour at room temperature or 37°C. Alternatively, add 300 μL of blocking buffer to each well, then immediately invert the plate to empty contents. Repeat this process two more times.

3.Proceed with the ELISA protocol that is appropriate for your downstream detection. For storage, invert plate for approximately 2 hours to dry. Transfer plate to a plastic bag or other container containing a desiccant, such as silica gel. Store the plate at 4°C.