AsurDx™ Biotin (Vitamin H) ELISA Test

The AsuDxTM Biotin (Vitamin H) ELISA Test kit enables government agencies, food manufacturers, and quality assurance organizations to detect Biotin in vitamin powder/tablets, milk, milk powder, and vitamin juice samples to satisfy customer concerns about food safety.


  • Easy, convenient, and time-saving method to measure the level of Biotin in vitamin powder/tablets, milk, milk powder, and vitamin juice;
  • Detection Range: 1 – 50 ng/ml;
  • Sensitivity: < 0.05 ng/ml
  • Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10% No significant cross-reactivity or interference was observed
  • High reproducibility

*Availability/Distribution: The product is designed and developed by BioStone US Texas headquarters and manufactured/assembled by BioStone oversea subsidiaries or partners. Currently, the product is only available outside of the USA. Regulatory requirements vary by oversea countries; the product may not be available in your geographic area.


MethodCompetitive ELISA
Coated AntigenBiotin-coated Plate
Incubation Time75 minutes
StorageAt least 12 months
SpecificityVitamin Powder/Tablets, Milk, Vitamin Juice

Order Information

Catalog Number2010-02
Plates2 plates
Plate Format12 X 8-well strips

Product Description

Biotin, also known as vitamin H, is an important nutritional factor and feed additive, playing a major role in the metabolism of both humans and animals alike. Biotin acts as a coenzyme and participates in the process of gluconeogenesis, biosynthesis of fatty acids, and the metabolism of amino acids. Biotin deficiency in mammals leads to growth inhibition, loss of appetite, and reduced utilization of food components. Advanced stages of avitaminosis manifest themselves in hair loss, dermatitis, and signs of paralysis. The biotin requirement for adults is between 30 and 60 μg per day.

The AsuDxTM Biotin (Vitamin H) ELISA Test Kit is a competitive ELISA assay for the quantitative measurement of Biotin in vitamin powder/tablets, milk, milk powder, and vitamin juice. The density of color is proportional to the amount of Biotin captured from the samples.