AsurDx™ TMB Substrate
AsurDx™ TMB Substrate can be used for peroxidase-based enzyme immunoassays. Upon oxidation, 3,3’,5,5’‐tetramethylbenzidine (TMB) forms a water-soluble blue reaction product that can be measured spectrophotometrically at 605-650 nm. Upon acidification, the reaction product becomes yellow with an absorbance peak at 450 nm.
*Warning: Read the Safety Data Sheets (SDSs) and follow the handling instructions. Wear appropriate protective eyewear, clothing, and gloves. For Research Use Only. Not for use in diagnostic procedures.
Features of TMB Substrate Solution
- Ready‐to‐use one-component solution
- Outstanding Signal Generation
- Reliable Durability
Storage
Shipped on cold packs. Store at 2-8°C immediately upon receipt. Do not use product beyond the stated expiration date.
Precautions
This product is sensitive to contamination from a variety of oxidizing agents. To avoid contamination and premature expiration, avoid contacting TMB solution with any potential source of contamination. Never pipette directly from the bottle. Pour out required amount onto a tube and pipette from the tube. Do not return excess TMB to primary storage container.
Product List
| Product Name | Volume | Catalog Number | Description | |
| AsurDxTM ELISA Blocking Buffer I | 30mL | BAH-T08430 | Tetramethylbenzidine was dissolved in citrate buffer. Hydrogen peroxide was added to the solution. | |
| AsurDxTM TMB Substrate | 60mL | BAH-T08431 | Tetramethylbenzidine was dissolved in citrate buffer. Hydrogen peroxide was added to the solution. | |
| AsurDxTM TMB Substrate | 1 L | BAH-T08432 | Tetramethylbenzidine was dissolved in citrate buffer. Hydrogen peroxide was added to the solution. |
Procedure
1. Add 100 µL of TMB substrate to each well-containing peroxidase reaction.
2. Incubate at room temperature for 10 to 20 minutes.
3. Add 100 µL of stop solution) to each well.
4. Measure the absorbance at 450nm.