AsurDx™ Bovine Viral Diarrhea Virus (BVDV) Ag Test Kit
The AsurDxTM Bovine Viral Diarrhea Virus (BVDV) Ag Test Kit is designed for the detection of Bovine Viral Diarrhea Virus (BVDV) non-structural protein p80/p125 antigen in bovine whole blood (with heparin or EDTA), plasma, serum, leucocytes or ear notch samples.
- Detects BVDV in in bovine whole blood plasma, serum, leucocytes or ear notch samples;
- Detects both BVDV type 1 and 2 strains in the PI cattle, with restriction for transient viraemic animals which are not systematically detected;
- Procedures last less than 2 hours;
- Provides a simple, rapid, sensitive and cost-effective enzyme-based immunoassay (ELISA) screening method
*Availability/Distribution: Product is designed and developed by BioStone US Texas headquarter and manufactured/assembled by BioStone oversea subsidiaries or partners. Currently, the product is only available outside of the USA. Regulatory requirements vary by oversea countries; the product may not be available in your geographic area.
|Method||Colorimetric sandwich ELISA|
|Coated Antigen||Anti-p80/p125 Ab-coated Plate|
|Incubation Time||2 hours|
|Storage||At least 12 months|
|Specificity||both BVDV type 1 and 2 antigens in the PI cattle|
|Plates||2 plates||5 plates|
|Plate Format||12 X 8-well strips||12 X 8-well strips|
The BVDV is capable of producing a broad range of clinical signs, ranging from most often asymptomatic infection to severe acute disease with signs from the enteric, reproductive or respiratory organs. Bovine fetus infected with non-cytopathic biotype of BVDV between days 30 and 125 of gestation can develop immune tolerance against the virus and will be born persistently infected (PI) shedding the virus continuously. Bovine viral diarrhea virus (BVDV) suppresses a cow’s immune system, making the animal susceptible to a host of other infections. BVDV is one of the world’s most costly bovine diseases, with losses from reduced herd productivity, health and reproductive efficiency.
One of the most immunogenic proteins of BVDV is non-structural NS2/3 (p80/p125), which is essential for the BVDV viral RNA replication and cytopathogenicity. This protein is also highly conserved among pestiviruses and induces a strong humoral immune response in cattle exposed to live BVDV. Therefore, p80/p150 protein is a proper antigen target to detect two major antigenically distinct genotypes of BVDV 1 and 2 in the persistently infected (PI) cattle.
PI animals may be identified by a variety of techniques such as virus isolation, polymerase chain reaction (PCR) or immunohistochemistry (IHC). These methods are effective but they require skilled personnel and highly equipped laboratories.